Comparison of Natural Dyes from Telang Flower Extracts (Clitoria ternatea L) as a Substitute for Methylen Blue in Diff Quik Painting Perbandingan Pewarna Alami dari Ekstrak Bunga Telang (Clitoria ternatea L) sebagai Pengganti Methylen Blue pada Pengecatan Diff Quik
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Published:
Jul 8, 2021
Keywords:
Telang Flower, Natural Dyes, Citology
Main Article Content
Abstract
Telang flower (Clitorea ternatea L) is a type of vine that grows wild in rice fields and has been widely used as food coloring. Telang flowers contain several active compounds, which is anthocyanin gives a blue color. This research was conducted with the aim of knowing the telang flower extract as a natural dye to substitute methylenen blue in diff quik painting. The analysis samples used were oral mucosal ephithelial cells which were taken by scraping the oral mucosa using cytobrush. Dried and mashed telang flower was then dissolved with distilled water (1:5) to make an extracts before the trial process of the samples. The results showed that the epithelial cells stained with methylene blue were better than telang flower extract. The immersion time of 15 minutes gave different colors to the cell nucleus and cytoplasm compared to 2 minutes and 5 minutes of immersion.
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How to Cite
[1]
E. N. Azka, A. A. Mandasari, and S. D. Santoso, “Comparison of Natural Dyes from Telang Flower Extracts (Clitoria ternatea L) as a Substitute for Methylen Blue in Diff Quik Painting”, PELS, vol. 1, no. 2, Jul. 2021.
Section
Agricultural Product Technology
References
[1] A. Abdullah and A. Apriandi, “Aktivitas antioksidan dan komponen bioaktif keong Ipong-ipong (Fasciolaria salmo),” J. Pengolah. Has. Perikan. Indones., vol. 14, no. 1, 2011.
[2] A. Suebkhampet and P. Sotthibandhu, “Effect of using aqueous crude extract from butterfly pea flowers (Clitoria ternatea L.) as a dye on animal blood smear staining,” Suranae J. Sci. Technol., vol. 19, no. 1, pp. 15–19, 2012.
[3] B. J. Bain, Blood cells: a practical guide. John Wiley & Sons, 2014.
[4] F. HECKNER, M. FREUND, and S. I. WANANDI, “Atlas Hematologi: Praktikum Hematologi dengan Mikroskop (Praktikum der mikroskopischen Hamatologie),” 1999.
[5] I. Fathoni, “PEMANFAATAN BENTONIT TEKNIS SEBAGAI ADSORBEN ZAT WARNA,” UNESA J. Chem., vol. 5, no. 3, 2016.
[6] J. M. Bueno, P. Sáez-Plaza, F. Ramos-Escudero, A. M. Jiménez, R. Fett, and A. G. Asuero, “Analysis and antioxidant capacity of anthocyanin pigments. Part II: chemical structure, color, and intake of anthocyanins,” Crit. Rev. Anal. Chem., vol. 42, no. 2, pp. 126–151, 2012.
[7] K. S. Budiasih, “Kajian potensi farmakologis bunga telang (Clitoria ternatea),” in Prosiding Seminar Nasional Kimia UNY, 2017, vol. 21, no. 4, pp. 183–188.
[8] L. Abramian and H. El-Rassy, “Adsorption kinetics and thermodynamics of azo-dye Orange II onto highly porous titania aerogel,” Chem. Eng. J., vol. 150, no. 2–3, pp. 403–410, 2009.
[9] M. A. Hartono, E. M. E. Purwijantiningsih, and S. Pranata, “Pemanfaatan ekstrak bunga telang (Clitoria ternatea L.) sebagai pewarna alami es lilin,” Fak. Teknobiologi Univ. Atma Jaya Yogyakarta, 2013.
[10] N. Ahmadiani, R. J. Robbins, T. M. Collins, and M. M. Giusti, “Anthocyanins contents, profiles, and color characteristics of red cabbage extracts from different cultivars and maturity stages,” J. Agric. Food Chem., vol. 62, no. 30, pp. 7524–7531, 2014.
[11] O. Hamdaoui and M. Chiha, “Removal of Methylene Blue from Aqueous Solutions by Wheat Bran.,” Acta Chim. Slov., vol. 54, no. 2, 2007.
[12] O. G. Avwioro et al., “Curcuma longa extract as a histological dye for collagen fibres and red blood cells,” J. Anat., vol. 210, no. 5, pp. 600–603, 2007.
[13] O. R. Fennema and S. R. Tannenbaum, “Introduction to food chemistry,” FOOD Sci. Technol. YORK-MARCEL DEKKER-, pp. 1–16, 1996.
[14] S. A. Al-Tikritti and F. Walker, “Anthocyanin BB: a nuclear stain substitute for haematoxylin.,” J. Clin. Pathol., vol. 31, no. 2, p. 194, 1978.
[15] S. Wahyuni and V. R. Tosiyana, “Identifikasi preparat gosok tulang femur ayam (Gallus gallus) dengan pewarnaan alami bunga telang (Clitoria ternatea L.),” in Prosiding Seminar Nasional Pendidikan Biologi, 2018.
[16] V. Vinukumar, K. V Kalaranjini, B. Krishnaswamy, and S. Vasudevan, “Utility of automation in assessment of haematological profile and typing of anaemia in pre-school children--a tertiary centre study,” J. Evol. Med. Dent. Sci., vol. 3, no. 16, pp. 4310–4316, 2014.
[2] A. Suebkhampet and P. Sotthibandhu, “Effect of using aqueous crude extract from butterfly pea flowers (Clitoria ternatea L.) as a dye on animal blood smear staining,” Suranae J. Sci. Technol., vol. 19, no. 1, pp. 15–19, 2012.
[3] B. J. Bain, Blood cells: a practical guide. John Wiley & Sons, 2014.
[4] F. HECKNER, M. FREUND, and S. I. WANANDI, “Atlas Hematologi: Praktikum Hematologi dengan Mikroskop (Praktikum der mikroskopischen Hamatologie),” 1999.
[5] I. Fathoni, “PEMANFAATAN BENTONIT TEKNIS SEBAGAI ADSORBEN ZAT WARNA,” UNESA J. Chem., vol. 5, no. 3, 2016.
[6] J. M. Bueno, P. Sáez-Plaza, F. Ramos-Escudero, A. M. Jiménez, R. Fett, and A. G. Asuero, “Analysis and antioxidant capacity of anthocyanin pigments. Part II: chemical structure, color, and intake of anthocyanins,” Crit. Rev. Anal. Chem., vol. 42, no. 2, pp. 126–151, 2012.
[7] K. S. Budiasih, “Kajian potensi farmakologis bunga telang (Clitoria ternatea),” in Prosiding Seminar Nasional Kimia UNY, 2017, vol. 21, no. 4, pp. 183–188.
[8] L. Abramian and H. El-Rassy, “Adsorption kinetics and thermodynamics of azo-dye Orange II onto highly porous titania aerogel,” Chem. Eng. J., vol. 150, no. 2–3, pp. 403–410, 2009.
[9] M. A. Hartono, E. M. E. Purwijantiningsih, and S. Pranata, “Pemanfaatan ekstrak bunga telang (Clitoria ternatea L.) sebagai pewarna alami es lilin,” Fak. Teknobiologi Univ. Atma Jaya Yogyakarta, 2013.
[10] N. Ahmadiani, R. J. Robbins, T. M. Collins, and M. M. Giusti, “Anthocyanins contents, profiles, and color characteristics of red cabbage extracts from different cultivars and maturity stages,” J. Agric. Food Chem., vol. 62, no. 30, pp. 7524–7531, 2014.
[11] O. Hamdaoui and M. Chiha, “Removal of Methylene Blue from Aqueous Solutions by Wheat Bran.,” Acta Chim. Slov., vol. 54, no. 2, 2007.
[12] O. G. Avwioro et al., “Curcuma longa extract as a histological dye for collagen fibres and red blood cells,” J. Anat., vol. 210, no. 5, pp. 600–603, 2007.
[13] O. R. Fennema and S. R. Tannenbaum, “Introduction to food chemistry,” FOOD Sci. Technol. YORK-MARCEL DEKKER-, pp. 1–16, 1996.
[14] S. A. Al-Tikritti and F. Walker, “Anthocyanin BB: a nuclear stain substitute for haematoxylin.,” J. Clin. Pathol., vol. 31, no. 2, p. 194, 1978.
[15] S. Wahyuni and V. R. Tosiyana, “Identifikasi preparat gosok tulang femur ayam (Gallus gallus) dengan pewarnaan alami bunga telang (Clitoria ternatea L.),” in Prosiding Seminar Nasional Pendidikan Biologi, 2018.
[16] V. Vinukumar, K. V Kalaranjini, B. Krishnaswamy, and S. Vasudevan, “Utility of automation in assessment of haematological profile and typing of anaemia in pre-school children--a tertiary centre study,” J. Evol. Med. Dent. Sci., vol. 3, no. 16, pp. 4310–4316, 2014.