RAPD Markers Distinguish Ground Beetle Species Using Genetic Polymorphism
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Published:
May 27, 2024
Keywords:
RAPD Markers, Genetic Polymorphism, Ground Beetles, Insect Classification, Molecular Genetics
Main Article Content
Abstract
This study focuses on differentiating between two ground beetle species, Oryctes rhinoceros and Allomyrina dichotoma, using RAPD markers due to the limitations of phenotypic methods. Ten RAPD primers were employed to identify polymorphic markers. Results showed a total of 41 polymorphic bands, with primers OP-C06 and OP-C15 displaying significant variation in polymorphism percentages, 100% and 16.7% respectively. Primer OP-A08 demonstrated the highest efficiency rate (17.07%) and effectively distinguished between the two beetle species, unlike primers OP-B09, OP-B14, and OP-C10, which showed zero polymorphism. This study underscores the utility of RAPD markers, particularly OP-A08, in accurately identifying insect species, thereby advancing genetic classification techniques.
Highlights:
- Effective Distinction: OP-A08 distinguished Oryctes rhinoceros and Allomyrina dichotoma.
- High Efficiency: OP-A08 had the highest efficiency rate at 17.07%.
- Reliable Classification: RAPD markers outperform phenotypic methods for insect identification.
Keywords: RAPD Markers, Genetic Polymorphism, Ground Beetles, Insect Classification, Molecular Genetics
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How to Cite
[1]
A. T. Humadi, A. A. Sultan, and S. W. Khaled, “RAPD Markers Distinguish Ground Beetle Species Using Genetic Polymorphism”, PELS, vol. 7, pp. 717-729, May 2024.
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Agricultural Product Technology
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
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[3] R. S. Prosser, J. C. Anderson, M. L. Hanson, K. R. Solomon, and P. K. Sibley, "Indirect Effects of Herbicides on Biota in Terrestrial Edge-of-Field Habitats: A Critical Review of the Literature," Agriculture, Ecosystems & Environment, vol. 232, pp. 59-72, 2016, doi: 10.1016/j.agee.2016.07.009.
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[9] P. R. Nair, A. S. Sukumaran, Murali, and C. Mohan, "Rhinoceros Beetle (Oryctes Rhinoceros L.) and Its Bio Control Agents," Technical Bulletin No. 43, Central Plantation Crops Research Institute, Kayamkulam, Kerala, India, 2002.
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[12] Z. Kui, J. F. Chen, and Y. X. Cai, "Cultivation of Medicinal Insect of Japanese Rhinoceros Beetles," Zhong Cao Yao, vol. 31, pp. 633-634, 2000.
[13] N. A. Ratcliffe, C. B. Mello, E. S. Garcia, T. M. Butt, and P. Azambuja, "Insect Natural Products and Processes: New Treatments for Human Disease," Insect Biochemistry and Molecular Biology, vol. 41, pp. 747-769, 2011, doi: 10.1016/j.ibmb.2011.05.007.
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[20] A. J. Al-Karawi and A. A. Sultan, "Molecular Classification for Some Species of Ground Beetles to the Carabidae Family Spread in Diyala Governorate," Texas Journal of Agriculture and Biological Sciences, vol. 5, pp. 43-53, 2022.
[21] S. S. Hamad, "Molecular Genetic Study and Gene Expression of Cytochrome P450 Between Tribolium Castaneum and Tribolium Confusum Responsible for Different Pesticides," M.S. thesis, College of Education for Pure Sciences, University of Diyala, Iraq, 2022.
[22] S. R. Skoda, J. L. Figarola, S. Pornkulwat, and J. E. Foster, "Inter- and Intra-Specific Identification of the Screwworm, Cochliomyia Hominivorax, Using Random Amplified Polymorphic DNA-Polymerase Chain Reaction," Journal of Insect Science, vol. 13, no. 76, pp. 1-15, 2013.
[23] J. G. K. Williams, A. R. Kubelik, K. J. Livak, J. A. Rafalski, and S. V. Tingey, "DNA Polymorphisms Amplified by Arbitrary Primers are Useful as Genetic Markers," Nucleic Acids Research, vol. 18, pp. 6531-6535, 1990.
[24] K. P. Williams and D. P. Bartel, "PCR Product with Strands of Unequal Length," Nucleic Acids Research, vol. 23, no. 20, pp. 4220-4221, 1995.
[25] A. Sultan, "Comparative Molecular Study Between Two Species of Flour Beetle Tribolium Castaneum and Tribolium Confusum by Using RAPD-PCR," Systematic Reviews in Pharmacy, vol. 11, no. 12, pp. 1604-1609, 2020.